Some of these steps will be redundant but it may help others.
First, use a programming cable with a genuine FTDI chip. 99% of problems with software not reading the radios come from the prolific chip cables.
Second, ALWAYS read from the radio first. If it is the first time use, save the resulting file if you ever need it later. (None of the frequencies that come loaded into the radios from the factory are legal to use in North America however.)
Next, the UV-5RTP is a tri-power radio, and the UV-5R setting in Chirp might not work properly. Try the Baofeng BF-F8HP instead. It might work. If not, use the factory software.
If you can get Chirp to work, populate the fields like so, left to right:
Frequency = 466.1125
Name = Channel 1
Tone mode= DTCS
DTCS code = 025
DTCS Pol = NN
Duplex = None
Mode = NFM
Power = High
All other fields in Chirp should be blank.
Now populate the fields for channel 2 and 3 the same, except use the appropriate frequencies and DTCS codes. You will know you are using the correct PL tones because the drop box will list the numbers 025, 411 and 516 (along with about 60 others.)
Save the program and write to the radios. All will now be fine.